Half-life & dosing frequency

Half-life numbers look intimidating on a compound page, but the whole concept comes down to one practical question: how often should I dose this? Here’s how to read a half-life and translate it into a frequency that makes sense.

What “half-life” actually means

A compound’s half-life is the time it takes for the body to eliminate half of what’s currently circulating. After one half-life, 50% of the dose is gone. After two half-lives, 75%. After three, 87.5%. After four, about 94%.

It’s not a clock that resets — it’s a percentage rule. So a compound with a 4-hour half-life doesn’t disappear in 4 hours; it just halves every 4 hours, asymptotically approaching zero.

The practical version: after about four half-lives, the dose is essentially cleared (~94%). After five, it’s gone for practical purposes (~97%). That’s the threshold most research protocols use when deciding how often to re-dose.

The four-half-lives rule

Most dosing protocols are designed around two competing goals:

  • Maintain a useful plasma concentration — you need enough of the compound in circulation to engage its target.
  • Avoid runaway accumulation — dosing faster than the body clears means levels stack up across days and eventually overshoot.

Half-life sets the balance point. If you re-dose around one half-life later, you maintain a roughly steady plasma level. If you wait four or five half-lives between doses, you’re letting the compound clear almost completely between hits — which suits short-acting compounds where you actually want a peak-and-trough pattern.

A short half-life vs a long one — visualised

Plasma concentration over 7 days · once-daily dosing BPC-157 (4h half-life) Daily dose, fast clearance — sharp daily spikes Day 1 Day 3 Day 5 Day 7 peak ~0 TB-500 (~48h half-life) Daily dose, slow clearance — accumulates to steady state Day 1 Day 3 Day 5 Day 7 peak ~0 Same daily dose. Very different plasma profile. BPC-157 returns to baseline between doses. TB-500 builds toward a steady state over 5–7 days. That’s why TB-500 is often dosed less frequently (2× per week) — daily dosing accumulates faster than receptor signalling can use.
Two compounds, identical dosing schedule, very different pharmacokinetics.

Translating half-life into a dosing schedule

Re-dose interval ≈ half-life  →  steady plasma concentration

Re-dose interval ≈ 4 × half-life  →  peak-and-trough pattern, near-complete clearance

A few worked examples:

CompoundHalf-lifeCommon dosing in researchWhy
BPC-157~4 hoursOnce or twice dailyClears almost fully overnight — daily dosing produces a fresh peak each day rather than accumulating
TB-500~48 hoursTwice weeklyDaily dosing accumulates faster than receptor signalling can use; 2×/week maintains steady levels
Semaglutide~7 daysOnce weeklyHalf-life slightly longer than the dosing interval, so plasma sits at near-steady state
Retatrutide~6 daysOnce weeklySame logic as Semaglutide — weekly dosing matches the elimination window
IGF-1 LR3~20–30 hoursOnce dailyLong enough to bridge 24 hours, short enough that daily dosing doesn’t pile up
Ipamorelin~2 hours2–3 times dailyShort pulse with full clearance — needed to trigger natural GH-pulse pattern

Why some compounds break the rule

The half-life → frequency mapping works for most peptides, but a few categories deliberately break it:

Pulsatile-signalling compounds

GHRPs (Ipamorelin, GHRP-2, GHRP-6) work by triggering a pituitary pulse, not by sitting in plasma. They’re dosed several times a day even though their half-lives could support longer intervals — because the goal is repeated signal pulses, not maintained concentration.

Compounds with long downstream effects

Tesamorelin has a plasma half-life of ~25 minutes but is dosed once daily. The GH pulse it triggers and the IGF-1 elevation that follows last hours, so the practical effect outlives the parent compound by a wide margin.

Receptor-saturation compounds

Some compounds saturate their receptors at far lower concentrations than peak plasma levels. Once the receptors are saturated, additional plasma concentration doesn’t add effect. For these, dosing schedule is set by receptor recycling time, not plasma clearance.

Where to find the half-life for any compound: on the individual compound page in our peptide library. The figure is published on every research-grade COA and matches what you’ll find in pharmacokinetic literature.