Use this calculator to work out reconstitution volumes and per-administration draw amounts for TB-500 (Thymosin Beta-4 fragment). Enter your vial size, BAC water volume, and target dose — the calculator returns the exact volume in mL, syringe units to draw to, the concentration, and doses per vial.
For research and educational purposes only. This calculator performs volumetric mathematics on the values you enter; it does not recommend doses or provide medical advice.
Universal peptide & nootropic dose calculator
Standard research pen format: 3 mL total volume, 300 clicks per pen. That’s fixed — so the only thing that changes is how many mg are in your specific pen. mg per click = your pen’s mg ÷ 300.
Vial mode: for lyophilised powder vials you reconstitute yourself with bacteriostatic water. Tell us the vial mg, how much BAC water you added, and your target dose — we work out the exact volume to draw on a U-100 insulin syringe.
Research and educational purposes only. Not medical advice. Not for human consumption. This calculator performs volumetric mathematics on the values you enter; it does not recommend doses. Compounds listed are not licensed as medicines for human use unless stated otherwise.
About TB-500
TB-500 is a synthetic fragment of Thymosin Beta-4, a 43-amino-acid protein. The TB-500 fragment corresponds to the active region of the parent molecule. It is supplied as a research-grade lyophilised powder and is studied in tissue-repair and angiogenesis research literature. TB-500 is unscheduled under the UK Misuse of Drugs Act 1971 and is not licensed as a medicine in the UK or any other jurisdiction.
In published research literature, TB-500 has been examined in studies relating to tendon, ligament and muscle tissue repair, cellular migration, and wound-healing pathways. It is commonly stacked with BPC-157 (the Wolverine research stack) in published recovery protocols.
Typical reconstitution / pen formats
| Format | BAC water | Concentration / mg per click | Volume to draw / clicks | For |
|---|---|---|---|---|
| 5mg vial | 2 mL BAC water | 2.5 mg/mL | 0.10 mL (10 units U-100) | for a 2.5mg target administration |
| 10mg vial | 2 mL BAC water | 5.0 mg/mL | 0.05 mL (5 units U-100) | for a 2.5mg target administration |
| 10mg vial | 5 mL BAC water | 2.0 mg/mL | 0.125 mL (12.5 units U-100) | for a 2.5mg target administration |
TB-500 dosing observed in research literature
Reported research-literature dose ranges for TB-500 in subcutaneous administration to laboratory animals fall predominantly in the 2–2.5 mg range per administration, with administration typically twice per week for the first 4–6 weeks of a research protocol, followed by a single weekly administration as a maintenance schedule. Total weekly TB-500 of 5mg is the commonly-described starting protocol.
These figures are reported here for completeness of the research-literature picture and do not constitute a dosage recommendation.
Half-life and frequency
TB-500’s longer subcutaneous half-life (reported at 2–4 days in animal studies) is what enables the weekly or twice-weekly research-administration pattern. This is meaningfully longer than BPC-157, which is why combined Wolverine-stack protocols often run BPC-157 daily alongside TB-500 weekly.
Storage and shelf life
Lyophilised TB-500 powder is stable refrigerated at 2–8°C for 12–24 months unopened, longer at -20°C. Reconstituted with bacteriostatic water it is stable refrigerated for approximately 28 days. With sterile water (no preservative) only 24–48 hours.
Frequently asked questions
How much bacteriostatic water do I add to a 5mg TB-500 vial?
Most research protocols use 2 mL of bacteriostatic water for a 5mg vial, giving a concentration of 2.5 mg/mL. This produces a 10-unit draw on a U-100 insulin syringe for a 2.5mg target administration.
How often is TB-500 administered in research?
Most published research protocols describe twice-weekly administration for 4–6 weeks (the loading phase), then once-weekly maintenance. Some protocols use a single weekly dose throughout.
What is the Wolverine stack?
The Wolverine stack is a combined BPC-157 + TB-500 research protocol. Both compounds are studied in tissue-repair contexts; the stack is the most-researched recovery combination in published literature. See our Wolverine stack page for the multi-compound calculator.
How long does TB-500 last after reconstitution?
Approximately 28 days refrigerated when reconstituted with bacteriostatic water. With sterile water (no preservative) only 24–48 hours.
Is TB-500 legal in the UK?
TB-500 is not a controlled substance under the UK Misuse of Drugs Act 1971. It is sold as a research-grade compound for laboratory use only. It is not licensed as a medicine and is not approved for human consumption.
What is the difference between TB-500 and Thymosin Beta-4?
TB-500 is a synthetic fragment of the larger Thymosin Beta-4 protein. The TB-500 fragment corresponds to the active region of TB4 that is most-studied in research. The names are often used interchangeably in research literature, though they are not chemically identical.
Related compounds and stacks
Where to source research-grade TB-500
For UK readers researching TB-500, our partner supplier stocks research-grade preparations. (Partner link to be added after the affiliate arrangement is signed.) We do not sell peptides ourselves and are not responsible for any third-party supplier’s product quality, regulatory compliance, or customer service.
Compliance: Stackcalc.co.uk provides calculator tools and information for research and educational purposes only. We do not sell, supply, or recommend peptides, nootropics, or any other compounds. Content on this site does not constitute medical advice. Always consult a qualified healthcare professional. Not for human consumption.